FIG 5.
A cluster of four amino acids determines the functionality of CD300LF as an MNV receptor. (A and C) Analysis of MNV replication in BV2-KO cells transduced with the indicated mutant alleles of CD300LF. Cells were inoculated with MNV at an MOI of 5 TCID50/cell and harvested at 24 h postinfection to determine the titer of infectious viruses via TCID50 assay in BV2 cells. All experiments were done in triplicates, and data are presented as mean ± SEM. N.D., not detected. (B and D) Analysis of surface expression of HA-tagged CD300LF mutants in the indicated cells. Data shown are representative plots from three independent experiments. Asterisks indicate significance compared to B6/CD300LF. ns, not significant.