FIG 3.

NKILA inhibits HIV spreading replication. (A) Relative NKILA mRNA expression in MT4 cells expressing NKILA. MT4 cells were infected with lentiviruses containing NKILA or a scramble control, and at 48 h postinfection, puromycin (1 g/ml) was added to the medium for selection. (B) Two nanograms, 0.2 ng, or 0.02 ng p24 antigen of NL4-3 viral particles was added to infect the NKILA MT4 overexpression or control cell lines. Cells were washed with PBS three times after 4 h of infection. The supernatant p24 release amounts were analyzed by ELISA at 1 day, 3 days, 5 days, 7 days, and 9 days.