FIG 5.

HPV18 E7 binding to PTPN14 contributes to the repression of differentiation induced by suspension and improves survival of cells grown in suspension. N/Tert-1 keratinocytes expressing HPV18 E7 WT, HPV18 E7 R84S, or the empty vector control were induced to differentiate by culturing in ultralow adherence plates for 12 h. (A) qRT-PCR was used to measure the expression of the differentiation marker IVL relative to GAPDH in adherent or suspended cells. Graph displays the mean ± SD of three independent replicates. (B) Survival after suspension was assessed by replating 1,000 cells from suspension, allowing cells to grow for 5 days, and measuring total viable cell area by crystal violet staining. Graph displays the mean ± SD of three independent replicates. Representative images of crystal violet staining are displayed. Statistical significance was determined by ANOVA (*P < 0.05, ***P < 0.001).