FIG 1.

Optimized EBV genome sequencing from tumors and primary clinical samples. (A) Optimization results of various dNTP concentrations in mlrPCR-sWGA reaction measured as the EBV copy increase normalized by the overall DNA increase. (B) Incubation buffer (TE [Tris-EDTA] or Q solution), time (8 or 16 h), and temperature optimization for better EBV copy increase. (C) Overview of sample collection and methods for sequencing virus from Kenyan children diagnosed with eBL and healthy children as controls. Hybrid capture was universally performed along with additional amplification and enrichment steps to overcome small amounts of virus and input DNA. mlrPCR-sWGA, multiplexed long-range PCR–specific whole-genome amplification.