FIG 12.

The BHLF1 locus is uniformly transcribed during Lat III. Single-cell RNA-seq analysis of the EBV transcriptome in 10 cells of the B LCL GM12878, immortalized by the B95.8 isolate of EBV, indicated that BHLF1 transcripts were present in each cell. This was in contrast to the heterogeneous detection of transcripts encoded by known latency-associated genes, e.g., EBNA1, LMP2A/2B, EBNA2, and LMP1 (note that transcripts from the latter two genes are not evident in this figure due to the scale used for the vertical axis). Other peaks represent various lytic-cycle transcripts. The results shown were obtained by analysis, as described previously (81), of publicly available data sets from https://www.encodeproject.org/experiments/ENCSR673UIY/ (72).