FIG 1.

Induction of rod-shaped structures by HSV-1 gI. (A) Diagram of domain organization of HSV-1 KOS strain glycoprotein gI. (B and C) Vero cells were transfected to express HSV-1 gI-Myc, gI-GFP, and untagged gI. At 18 to 24 h posttransfection, the cells were fixed, permeabilized, and revealed by antibodies specific for the Myc tag, gI antigen, and HA tag or by GFP fluorescence. DAPI was used to stain cell nuclei (blue). The representative images were captured with a Leica confocal microscope and processed by using ImageJ. The percentage of cells showing rod-shaped structures relative to total gI-positive cells is also shown in the pictures. (D) HaCaT cells grown on coverslips were infected with WT HSV or the mutant gI P184&188S at an MOI of 0.1 at 37°C. After 1 h of incubation, the cells were rinsed with PBS and supplemented with fresh infection medium. At 24 h postinfection, the cells were fixed and stained with rabbit polyclonal antibodies against gI and mouse monoclonal antibodies against gE (red) as indicated. The rest of the steps were the same as panels B and C.