FIG 6.

Analysis of HSV-1 gI self-interaction. Vero cells were transfected to either singly express (A) or coexpress gI-GFP and gI-MT-myc, or gD-MT-myc (B) or gI-myc (C). The singly expressed proteins served as a control. At 18 to 24 h posttransfection, the cells were fixed, permeabilized, and stained with antibodies specific for Myc tag (red). The representative images were captured with a Leica confocal microscope and processed by using ImageJ. (D) Vero cells were transfected to express gI-HA and gI-myc or to singly express gI-myc. At 24 h posttransfection, the cells were lysed, and gI was immunoprecipitated with antibodies to HA epitope. The proteins bounded to the beads were separated by SDS-PAGE, followed by Western blotting with antibodies to either HA or c-myc epitope.