FIG 8.

The gI extracellular domain is the critical determinant for inducing rod-shaped structures. (A) Diagram of gI/gD chimeric mutants and gI CT truncated mutants. The corresponding SP, ECD, and TM regions were swapped in a reciprocal manner. (B and C) Mutants were transiently expressed in Vero cells and were with antibodies to Myc and HA epitopes, followed with Alexa Fluor 488-conjugated secondary antibodies. The cell nuclei were stained with DAPI (blue). The representative images were captured with a Leica confocal microscope and processed by using ImageJ.