FIG 3.

The shaft and knob domains contributed to the superinfection resistance. (A) Expression of the truncated fragments of fiber-1 in LMH cells was detected by IFA using a monoclonal antibody against Flag. (a to f) LMH cells were transfected with pcDNA3.1-F1-tail-Flag, pcDNA3.1-F1-shaft-Flag, pcDNA3.1-F1-knob-Flag, pcDNA3.1-F1-tail+shaft-Flag, pcDNA3.1-F1-shaft+knob-Flag, and pcDNA3.1, respectively. (B) Supernatants from infected LMH cells at different time points, which were transfected with pcDNA3.1-F1, pcDNA3.1-F1-tail-Flag, pcDNA3.1-F1-shaft-Flag, pcDNA3.1-F1-knob-Flag, pcDNA3.1-F1-tail+shaft-Flag, pcDNA3.1-F1-shaft+knob-Flag, and pcDNA3.1 in advance, were titrated with TCID₅₀. (C) Infected LMH cells at 120 hpi, which were transfected with pcDNA3.1-F1 (lane 1), pcDNA3.1-F1-tail-Flag (lane 2), pcDNA3.1-F1-shaft-Flag (lane 3), pcDNA3.1-F1-knob-Flag (lane 4), pcDNA3.1-F1-tail+shaft-Flag (lane 5), pcDNA3.1-F1-shaft+knob-Flag (lane 6), and pcDNA3.1 (lane 7) in advance, were analyzed by Western blotting using MAb 1B4 against the hexon protein of FAdV-4. These experiments were performed three times with similar results.