FIG 5.

The regions of the FMDV IRES that interact with hnRNP K. (A) Predicted RNA secondary structure of the FMDV IRES. The M-FOLD program was used to predict the RNA secondary structure. (B) Map of the hnRNP K interaction region in the FMDV IRES. The purified recombinant protein GST-hnRNP K was mixed with RNA probes spanning different regions of the FMDV IRES. The RNA-protein complexes were purified with streptavidin beads, resolved by SDS-PAGE, and used in Western blot assays with an anti-GST antibody.