TABLE 1.

Oligonucleotides used in this study

Primer use	Sequence (5′→3′)	Targeta
Oligonucleotides for making targeted galK insertions into pp71	CGG AAG CGG CCG CGA TCA GCG AGG CCG AAG CCG CCA GCG GCA GCT TTG GTC CTG TTG ACA ATT AAT CAT CGG CA	pp71 C34S GalK FWD
	CGT AGA CGA CCG GCT TCC AGC GAG CCG CCT TCC ACG TTG GTG ATG AGC CGT CAG CAC TGT CCT GCT CCT T	pp71 C34S GalK REV
	AGG AGA ACA AAT CTC CGC ACG ACA CCG TAG ACC TGA CCG ATC TAA ACA TCC CTG TTG ACA ATT AAT CAT CGG CA	pp71 C94S GalK FWD
	AGG CGT CGT GGG CCA AAG TTG TTG AGG TCC ACC AGT AGT CGG TCC TGT TCT CAG CAC TGT CCT GCT CCT T	pp71 C94S GalK REV
	TCG CCT TTC GCG CCG GCG CTA TCC CGC TGA CGT TGG TAG ACG CCC TGG AGC CTG TTG ACA ATT AAT CAT CGG CA	pp71 C218S GalK FWD
	ATG ATC CAT TGG CCT CGC TCG TCC GTC TCC GTT TTG TGG ATG TAC GTA TTT CAG CAC TGT CCT GCT CCT T	pp71 C218S GalK REV
Oligonucleotides for making S-nitrosylation-resistant mutants (galK reversions)	CAG CGA GGC CGA AGC CGC CAG CGG CAG CTT TGG TCG CCT GCA CTC ACA GGT GCT TCG GCT CAT CAC CAA CGT GGA AGG CGG CTC GCT GG	pp71 C34S Mut FWD
	CCA GCG AGC CGC CTT CCA CGT TGG TGA TGA GCC GAA GCA CCT GTG AGT GCA GGC GAC CAA AGC TGC CGC TGG CGG CTT CGG CCT CGC TG	pp71 C34S Mut REV
	GCA CGA CAC CGT AGA CCT GAC CGA TCT AAA CAT CAA GGG CCG CTC AGT GGT GGG CGA ACA GGA CCG ACT ACT GGT GGA CCT CAA CAA CT	pp71 C94S Mut FWD
	AGT TGT TGA GGT CCA CCA GTA GTC GGT CCT GTT CGC CCA CCA CTG AGC GGC CCT TGA TGT TTA GAT CGG TCA GGT CTA CGG TGT CGT GC	pp71 C94S Mut REV
	CGC TAT CCC GCT GAC GTT GGT AGA CGC CCT GGA GCA GCT GGC CTC ATC GGA CCC TAA TAC GTA CAT CCA CAA AAC GGA GAC GGA CGA GC	pp71 C218S Mut FWD
	GCT CGT CCG TCT CCG TTT TGT GGA TGT ACG TAT TAG GGT CCG ATG AGG CCA GCT GCT CCA GGG CGT CTA CCA ACG TCA GCG GGA TAG CG	pp71 C218S Mut REV
Sequencing primers for confirming S-nitrosylation-resistant mutants	CGA CCT CCC ACG AAG ACC C	pp71 C34S Seq FWD
	GTC TAC GGT GTC GTG CGG AG	pp71 C34S Seq REV
	AGG TGC TTC GGC TCA TCA CC	pp71 C94S Seq FWD
	CCG CTG GCT CTG AAA GAG GT	pp71 C94S Seq REV
	CCG CAC GGC TCA CTA CTG G	pp71 C218S Seq FWD
	GGT GCG GGT ACG GAT TGT GT	pp71 C218S Seq REV
Primers for cloning pp71 (WT/C218S) into pCMSeGFP vector	TCG AGA ATT CGC CAC CAT GGA CTA CAA AGA CGA TGA CGA CAA GAT GTC TCA GGC ATC GTC CTC GC	EcoRI-Kozak-Flag-pp71 FWD
	AGC TGA ATT CCT AGA TGC GGG GTC GAC TGC GTG GGG TGC TGG AAG TGG AAG CGG TG	EcoRI-Kozak-Flag-pp71 REV
Primers for cloning pp71 (WT/C218S) into pLXSN vector	TCG AGA ATT CGC CAC CAT GTC TCA GGC ATC GTC CTC GCC CGG TGA GGG ACC CTC GTC GGA	EcoRI-Kozak-pp71 FWD
	AGC TGA ATT CCT AGA TGC GGG GTC GAC TGC GTG GGG TGC TGG AAG TGG AAG CGG TG	EcoRI-Kozak-pp71 REV
Real-time PCR primers	CAG CAA TTT TCA GTG TCA GAA GC	IFN-β1 FWD
	TCA TCC TGT CCT TGA GGC AGT	IFN-β1 REV
	GAA TGG GTT TGC TAG AAT GTG ATA	IL-1β FWD
	CAG ACT AGG GTT GCC AGA TTT AAC	IL-1β REV
	ATG GTT TTG CAG GCT TTG ATG	UL122 FWD
	ACC TGC CCT TCA CGA TTC C	UL122 REV
	CCC TCG GCT TCT CAC AAC AAT	UL54 FWD
	CGA GTT AGT CTT GGC CAT GCA T	UL54 REV
	GGT TTC TGG CTC GTG GAT GTC G	UL32 FWD
	CAC ACA ACA CCG TCG TCC GAT TAC	UL32 REV
	ACC CAC TCC TCC ACC TTT GAC	GAPDH FWD
	CTG TTG CTG TAG CCA AAT TCG T	GAPDH REV
	ACC ACT ACC TGA GCA CCC AGT C	eGFP FWD
	GTCCATGCCGAGAGTGATCC	eGFP REV
	CCC CTT CCA TCA CAT TGC A	mdm2 FWD
	AGT TTG GCT TTC TCA GAG ATT TCC	mdm2 REV

^aFWD, forward primer; REV, reverse primer.