FIG 1.

Schematic diagram of the genome organization of PEDV and positions of nucleotide changes made in icPEDV mutant viruses. (A) Schematic diagram of the genome organization of PEDV, with nonstructural proteins 1, 15, and 16 highlighted in gray. (B) Location of nucleotide sequence targeted for mutagenesis. The resulting change in the amino acid sequence is listed. For reference, Zhang et al. reported that the mutation F44A in Nsp1 reduced interferon antagonism in an overexpression system (16), and Hou et al. reported that mutation of Nsp16 in PEDV strain PC22A was attenuating in piglets (14). (See also Deng et al. [11].)