FIG 4.

Experimental design and outcomes after PEDV infection. (A) A total of 34 piglets from 3 sows were randomly grouped to 3 groups. These piglets were either mock infected or infected with icPEDV wild-type or icPEDV-mut4 virus. Blood samples were drawn prior to infection and also at 21 days after infection. (B) Clinical symptoms were evaluated and scored daily. The score is based on the status of feces and the overall appearance of each animal. Individual values and geometric means are presented. (C) Evaluating shedding of viral RNA. RNA was isolated from rectal swab samples and subjected to quantitative PCR to determine the number of genomic RNA copies per milliliter of sample. Individual values and geometric means are presented and were analyzed with unpaired t tests used for comparisons between the groups at the same time point. *, P < 0.05.