TABLE 2.
qPCR primer and probe sequences
| Method | Target | Primer sequence (5′–3′) |
|
|---|---|---|---|
| Forward | Reverse | ||
| SYBR green | Porcine IFN-β | AGCAGATCTTCGGCATTCTC | GTCATCCATCTGCCCATCAA |
| Porcine IFN-λ1 | ACTGTGATGCTGGACTTGG | GCATCCTTGGCTTTCTTGAAG | |
| Porcine GAPDH | ACCTCCACTACATGGTCTACA | ATGACAAGCTTCCCGTTCTC | |
| Porcine ISG54 | CTGGCAAAGAGCCCTAAGGA | CTCAGAGGGTCAATGGAATTCC | |
| Porcine N gene | CACTAACCTGGGTGTCAGAAA | CGTGAAGTAGGAGGTGTGTTAG | |
| Taqmana | PEDV N gene | GAATTCCCAAGGGCGAAAAT | TTTTCGACAAATTCCGCATCT |
a
The sequence of the N gene probe used in the Taqman assay was as follows: FAM-CGTAGCAGCTTGCTTCGGACCCA-BHQ (FAM, 6-carboxyfluorescein; BHQ, black hole quencher).