FIG 6.

Disruption of actin dynamics inhibits PRRSV infection via macropinocytosis. Both Cyto D and Lat A decreased PRRSV RNA abundance (A), N protein expression (B), and entry (C). The serum-starved MARC-145 cells were pretreated with Cyto D (20 or 40 μM), Lat A (0.125 or 0.25 μM), or DMSO and infected with PRRSV BJ-4 (MOI = 10) for 1 h. The cells were collected for assessment of PRRSV RNA abundance by RT-qPCR at 12 hpi, N protein expression by IB at 24 hpi, or binding and entry by RT-qPCR. Each experiment was performed three times, and similar results were obtained. Differences between groups were assessed by using a Student t test, and the statistical significance is indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, not significant).