FIG 1.
Generation and characterization of chimeric CSFVs. (A) Schematic diagram of the genomic organization of parental and chimeric viruses. Purple boxes indicate genes from the highly virulent Shimen strain, while red boxes indicate genes derived from the lapinized vaccine C-strain (also known as HCLV). The infectious clones of chimeric viruses (vSM-HCLVErnsE2DomainI, vSM-HCLVErnsE2DomainII, and vSME2L108P-I109T-HCLVErns) were developed based on the infectious clone of the non-rabbit-adaptive chimeric virus vSM-HCLVErns carrying the Erns glycoprotein of C-strain. (B) Indirect immunofluorescence staining of the PK-15 cells infected by chimeric viruses and parental virus. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI). Bar, 400 μm. (C) Growth kinetics of parental and chimeric viruses in PK-15 cells. The error bars represent the standard deviations for three replicates.