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Effects of mutations or deletion of sorting motifs on intracellular ORF3 protein transport. Vero cells grown on coverslips were transfected with pORF3wt and ORF3 mutant constructs including AITF, YLAG, YLAA, ALAI, AKL, AATI, YATG, EAA, AAD, and ΔYLAI. After 36 h of incubation, the cells were processed for IFA and stained for ORF3 protein, calnexin, and GM-130 as for Fig. 4. Scale bar represents 10 μm.
