TABLE 2.
Nucleotide sequences of oligonucleotide primers used for parental DR13 ORF3 (ORF3wt) gene synthesis
| Primer | Locationa | Sequence (5′–3′) |
|---|---|---|
| F1-F | 24785–24837 | GAATTCATGTTTCTTGGACTTTTTCAATACACGATTGACACAGTTGTCAAAGATGTCTC |
| F1-R | 24817–24875 | ACTCTTGGACAGCATCCAAAGACAAGTTGGCAGACTTTGAGACATCTTTGACAACTGTG |
| F2-F | 24855–24913 | CTTTGGATGCTGTCCAAGAGTTGGAGCTCAATGTAGTTCCAATTAGACAAGCTTCAAAT |
| F2-R | 24893–24951 | AAGTAGATAAAAACACTGGTGAAAAGAAAACCCGTAACATTTGAAGCTTGTCTAATTGG |
| F3-F | 24931–24989 | CACCAGTGTTTTTATCTACTTCTTTGCACTGTTTAAAGCGTCTTCTTTGCGGCGCAATT |
| F3-R | 24969–25027 | AAGAACGATGACAGCAAAACGCGCTGCCAACATAATATAATTGCGCCGCAAAGAAGACG |
| F4-F | 25007–25065 | CGTTTTGCTGTCATCGTTCTTTATTGCCCACTTTTATATTATTGTGGTGCATTTTTAGA |
| F4-R | 25045–25103 | AAAGCCTGCCAATAAGTGTGCAACAAATAATAGTTGCATCTAAAAATGCACCACAATAA |
| F5-F | 25083–25141 | GCACACTTATTGGCAGGCTTTGTTTAGTCTGCTTTTACTCCTGGCGCTATAAAAATGCG |
| F5-R | 25121–25179 | AGGAAAGAAAGTGTCGTAGTATTAAAGATAATAAAGAGCGCATTTTTATAGCGCCAGGA |
| F6-F | 25159–25217 | TACTACGACACTTTCTTTCCTCAATGGTAAAGCAGCTTATTATGACGGCAAATCCATTG |
| F6-R | 25197–25255 | GCCAAAAGTGATGTAATGGTCACCACCTTCTAGAATCACAATGGATTTGCCGTCATAAT |
| F7-F | 25235–25293 | GACCATTACATCACTTTTGGCAACTCTTTTGTTGCTTTCGTTAGTAGCATTGACTTGTA |
| F7-R | 25273–25331 | GATGTAGGTCAGCTTCTTGCCGCCCACGTATAGCTAGATACAAGTCAATGCTACTAACG |
| F8-F | 25311–25369 | GGCAAGAAGCTGACCTACATCTGTTGCGAACTGTTGAGCTTCTTGATGGCAAGAAGCTT |
| F8-R | 25349–25407 | TTAGTAATGCCGACAATTTGATGTTGCGAAAAGACATAAAGCTTCTTGCCATCAAGAAG |
| F9-F | 25387–25445 | TCAAATTGTCGGCATTACTAATGCCGCATTTGACTCAATTCAACTAGACGAGTATGCTA |
| F9-R | 25425–25477 | TTAACTACTAGACCATTACTCGAGTCATTCACTAATTGTAGCATACTCGTCTAGTTGAA |
a
Nucleotide positions are in accordance with the sequence of the virulent PEDV DR13 (GenBank accession no. JQ023161).