TABLE 3.
Primers used for plasmid constructions and rPEDV genome verification
| Primer | Location(s)a | Sequence (5′–3′)b |
|---|---|---|
| 1 | 24737–24786 | TGAAAAGGTCCACGTGCAGTGATGTTTCTTGGACTTTTTCAATACACGAT |
| 2 | 25513–25544 | GGTCAATTCGCATGTAAGACTTATAAACTCTA |
| 3F | 25342–25383 | GACTCAATTCAACTAGACGAGTATGCTACAATTAGTGAATGA |
| 3R | 25342–25383 | TCATTCACTAATTGTAGCATACTCGTCTAGTTGAATTGAGTC |
| 4 | 24726–24758 and 25343–25378 | TACGAAGCTTTTGAAAAGGTCCACGTGCAGTGAACTCAATTCAACTAGACGAGTATGCTACAATTAGTG |
| 5 | 25480–25500 | ATAGGTGTGTAAACTGCGCTA |
| 3F-XhoI | 24758–24779 | ATTCTCGAGATGTTTCTTGGACTTTTTCAAT |
| 3Rst-BamHI | 25362–25383 | ATTGGATCCCGTCATTCACTAATTGTAGCATAC |
| 3R-BamHI | 25362–25383 | ATTGGATCCCGTTCACTAATTGTAGCATAC |
a
The locations of primers are relative to the full genome sequence of the attenuated PEDV strain DR13 (GenBank accession no. JQ023162).
b
Restriction enzyme sites are marked by italic, bold letters. The stop codon is underlined.