FIG 5.

CHO-K1-derived stable cell lines expressing hDSG2 are more susceptible to HAdV55 infection than hCD46-expressing cells. (A) CHO-K1-derived stable cell lines expressing hDSG2. (B) hDSG2-expressing cell lines are susceptible to HAdV55 infection. (C) MFI of cells infected with HAdV55-EGFP. (D) CHO-K1-derived stable cell lines expressing hCD46. (E) hCD46-expressing cell lines are moderately infected by HAdV55. (F) MFI of cells infected with HAdV55-EGFP. (G) CHO-K1-derived stable cell lines simultaneously expressing hDSG2 and hCD46. (H) Cell lines expressing both hDSG2 and hCD46 are susceptible to HAdV55 infection. (I) MFI of cells infected with HAdV55-EGFP. Each stable cell line was stained with a FITC-labeled anti-hDSG2 antibody or a PE-labeled anti-hCD46 antibody and examined by flow cytometry. The cell lines were then infected with HAdV55-EGFP at 200 vp/cell. At 24 h postinfection, cells were imaged under a fluorescence microscope, and the expression of EGFP was examined by flow cytometry. Data are representative of those from three independent experiments. The MFI is presented as the mean ± SD. Comparison between groups was conducted by one-way ANOVA (n = 3). ***, P < 0.001.