Figure 1.

Significant DMR‐associated CpG sites and the fraction of DMRs localized to CpG islands, shelves, and shores. (a–b) Heat map representation of the hierarchical clustering of significant (p ≤ .05) DMR‐associated CpG sites in a) sperm (n = 15,991 CpGs) and b) blastocyst (n = 14,461 CpGs), where a positive z‐score corresponds to hypermethylation and a negative z‐score corresponds to hypomethylation relative to the mean. Samples in both sperm and blastocyst cluster into two distinct groups by young and APA samples. (c–d) The proportion of DMRs associated with CpG islands, shelves, and shores in c) sperm and d) blastocyst was tallied for hypermethylated and hypomethylated DMRs in APA relative to young. Hypomethylated CpG regions were significantly enriched in sperm but not blastocyst