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. 2020 Jul 21;19(8):e13195. doi: 10.1111/acel.13195

Figure 7.

Figure 7

The role of GDF15 in T‐cell activation and Th17 differentiation in vitro. (a) IL‐2 production from naïve CD4+ T cells stimulated with anti‐CD3 (2 μg/mL)/CD28 (5 μg/mL) in the absence or presence of varying concentrations of recombinant GDF15 for 72 hr. (b) IFN‐γ production from differentiated CD4+ T cells under Th1 culture conditions in the presence or absence of the indicated concentrations of recombinant GDF15. (c,d) Naïve lymph node T cells were stimulated with the indicated concentrations of anti‐CD3/CD28 and several concentrations of recombinant GDF15 for 24 hr and then analyzed for CD69 and CD25 expression by FACS. (e) The population of IL‐17+ cells under Th17 differentiation‐inducing culture conditions at the indicated concentrations of recombinant GDF15. (f) The transcription of RORγt in differentiated CD4+ T cells under Th17 differentiation‐inducing culture conditions at several concentrations of recombinant GDF15. Data are expressed as mean ± SEM ((a–f): one‐way ANOVA)