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. 2020 Aug 17;11:4133. doi: 10.1038/s41467-020-17955-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a GSEA enrichment scores for sets of genes differentially regulated in rat SCs treated with siCtcf or control siRNA. n = 3 independent experiments. b GSEA enrichment scores for genes involved in lipid biosynthetic process (left) and myelin sheath (right) in SCs treated with control or siCtcf. c GSEA enrichment scores for genes modified with H3K27me3 and for genes targeted by SUZ12, EZH2, or EED in SCs treated with control or siCtcf. d GSEA enrichment scores for genes upregulated genes in Eed cKO nerves from published data³⁷. NES, normalized enrichment score. e–g Immunoblotting for e PRC2 complex and CTCF proteins and for f, g histones with indicated modifications in SCs treated with control or siCtcf. e, f n = 2 independent experiments; g n = 3 independent experiments. h–j Co-immunoprecipitation of h, i HA-SUZ12 with Flag-CTCF from extracts of transiently transfected HEK293T cells or of j endogenous SUZ12 with CTCF from rat SCs. n = 2 independent experiments. k qRT-PCR analysis showing Suz12 and differentiation-related and myelin-related gene expression in rat SCs transfected with control siRNA or with Suz12-targeted siRNA (n = 3 independent experiments) and induced to differentiate for 9 h. Data are presented as means ± SEM., ***P < 0.001, **P < 0.01, *P < 0.05; two-tailed unpaired Student’s t-test, P_(Suz12) = 3.8E-05, P_(Egr2) = 3.3E-05, P_(Msn) = 0.0045, P_(Lss) = 0.0031, P _(Prx) = 1.5E-06, P_(Fasn) = 0.0019, P_(Hdac1) = 0.0019, P_(Sdc4) = 0.0015, P_(Srebf2) = 0.0026, P_(Mal) = 0.008, P_(Pllp) = 0.018, P_(Mag) = 0.017, P_(Notch3) = 0.012, P_(Hes1) = 0.02. Source data are provided as a Source Data file.