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. 2020 Aug 17;11:4133. doi: 10.1038/s41467-020-17955-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Heatmap of CTCF ChIP-seq peaks from proliferating and differentiated SCs. n = 1 in each condition. b ChIP-seq enrichment around TSS regions in proliferative and differentiated SCs. c Immunoblotting for H3K27me3 in proliferating and differentiating SCs. n = 2 independent experiments. d Enriched motifs of transcription factors (TF) in the CTCF-bound regions. e–g ChIP-seq enrichment around CTCF binding regions for e CTCF, and g H3K27ac in rat SCs, f H3K27me3 in rat sciatic nerves. h Heatmap for H3K27me3 and H3K27ac. The sites are ranked in ascending order of H3K27ac intensity. n = 1 in each condition. i GSEA enrichment for genes with H3K27me3 peaks associated with TSSs in siControl or siCtcf rat SCs. NES, normalized enrichment score. j Overlap between CTCF-bound and upregulated genes differentially expressed in rat SCs treated with control siRNA or siCtcf. k Heatmap of CTCF-targeted upregulated genes in rat SCs treated with control siRNA or siCtcf. n = 3 in each condition. l Tracks for the indicated genes with ChIP-seq of CTCF from rat SCs and of H3K27me3 from rat sciatic nerves. n = 1 in each condition with 20 million cells. m Expression of the indicated genes in RNA-seq dataset from rat SCs treated with control siRNA or siCtcf. n = 3 independent experiments, P_(Hes1) = 0.011, P_(Ccnd2) = 0.01007, P_(Rspo2) = 0.0105, P_(Shh) = 0.019, P_(Calca) = 0.041. n qRT-PCR for the indicated genes in rat SCs treated with control siRNA or siSuz12. n = 3 independent experiments, P_(Rspo2) = 0.006, P_(Shh) = 0.012, P_(Calca) = 0.02. o ChIP-qPCR for H3K27me3 at the promoters of the indicated genes in rat SCs treated with control siRNA or siCtcf. IgG were normalized to 1; n = 3 independent experiments, P_(Rspo2) = 0.039, P_(Shh) = 0.011, P_(Calca) = 0.039, P_(Sox2) = 0.0094. Data are presented as means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.