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. 2020 Aug 11;11:846. doi: 10.3389/fphys.2020.00846

FIGURE 2.

FIGURE 2

Overexpression of PRDM16 suppressed proliferation of ASMCs while enhancing apoptosis in AS mice. (A) EdU proliferation staining of cells in each group (×200, scale bar: 50 μm). (B) The relative fold of EdU positive cell in each group. (C) The apoptosis of cells in each group as detected by flow cytometry. (D) The apoptosis rate in each group as detected by flow cytometry. (E) Immunofluorescence of cells in each group (×200, scale bar: 50 μm), green staining was regarded as positive results. (F) The fluorescence intensity of CD31, α-SMA, and vimentin of cells in each group. (G) The cell migration in each group as detected by scratch assay. (H) The relative migration in each group. (I) The relative expression of miR-448 and PRDM16 as detected by RT-qPCR. (J,K) The expression of PRDM16 protein normalized to GAPDH as detected by Western blot analysis. *p < 0.05 compared with ASMCs transduced with oe-PRDM16 NC. The measurement data were expressed as mean ± standard deviation, and analyzed by independent-sample t-test, and the experiment was repeated three times independently.