Fig. 3. Characterization and impact of flow shear stress in SMCs isolated from wild-type (WT) and homozygous (HOZ) Lmna^G609G/G609G mice.

a Mouse SMCs were cultured for 9–26 days in arterial flow conditions (120 dyne/cm²). Immunofluorescence analyses performed on mouse SMCs (6-week-old WT and HOZ Lmna ^G609G/G609G mice) at passage 4 for α-SMA and Lamin A. Nuclei were stained with DAPI. Scale bar is 20 µm. n = 3–4 images examined over three independent experiments. b Percentage of dysmorphic nuclei, nuclei blebbing, and SMC organized fibers in mSMCs (assessed in static conditions). n = 3-4 images examined over three independent experiments. c Percentage of adhered cells over time. Cells were cultured under flow conditions. n = 3–4 independent experiments. Statistical analyses were performed by one-way ANOVA followed by Newman–Keuls’s post test. d Quantification of MMP13 in HOZ mSMCs and WT mSMCs. Cells were analyzed at day 0 and day 8 under flow. Fluorescence signal was normalized by cell number. n = 3–4 independent experiments. Statistical analyses were performed by a two-tailed unpaired Student’s t test. e Percentage of adhered cells over time. Cells were cultured under flow conditions. n = 5–6 independent experiments. In graphs b–e, results are mean ± SEM. *,**,***,**** denote statistical significance (p < 0.05, p < 0.01, p < 0.001, p < 0.0001).