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. 2020 Aug 17;9:F1000 Faculty Rev-991. [Version 1] doi: 10.12688/f1000research.25998.1

Table 2. Pros and cons of different vaccine formulations and examples of licensed vaccines.

Vaccine platforms Pros Cons Examples of licensed vaccines
targeted for humans
RNA •   Potential low-cost
manufacturing
•   Ease of manufacturing
•   Good safety profile
•   May have low immunogenicity
due to instability
•   May require multiple doses
-
DNA •   Potential low-cost
manufacturing
•   Ease of manufacturing
•   Good safety profile
•   Good stability
•   Does not induce anti-vector
immunity
•   Potential integration to human
genome
•   Low immunogenicity
-
Virus vectors
(replicating/non-
replicating viral vectors
and virus-like particles)
•   High-efficiency gene
transduction
•   High specific delivery of genes
to target cells
•   Induction of robust immune
responses
•   Increased cellular immunity
•   Low titer production
•   May induce anti-vector
immunity
•   Generation of replication-
competent virus, which can
induce tumorigenesis
•   JYNNEOS (Smallpox/
Monkeypox)
•   ACAM2000 (Smallpox)
•   Adenovirus type 4 and type 7
vaccine, live, oral (febrile acute
respiratory)
Inactivated •   Good safety profile
•   Can be used in
immunocompromised patients
•   Requires booster doses
•   Low production titer
•   Poliovax (Polio)
•   Flucelvax Quadrivalent
(Influenza)
•   Ixiaro (Japanese Encephalitis)
•   Imovax (Rabies)
Live attenuated virus •   High potency
•   Triggers long-lasting immunity
•   Low-cost manufacturing
•   Possible regression to virulence
strain
•   Limited use in
immunocompromised patients
•   ERVEBO (Ebola virus)
•   MMR II (Measles, Mumps, and
Rubella)
•   BCG vaccine (Tuberculosis)
Protein subunit •   Can be used in
immunocompromised patients
•   Good safety profile
•   Low immunogenicity
•   Conjugation could lead to
batch-wise variation
•   PedvaxHIB
( Haemophilus influenzae type b)
•   Engerix-B (Hepatitis B)
•   Recombivax HB (Hepatitis B)

This table was adapted from 811, 20, 2228.