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. 2020 Jul 28;21(15):5362. doi: 10.3390/ijms21155362

Figure 2.

Figure 2

Flavopereirine inhibited autophagic flux in MDA-MB-231 cells. Representative fluorescent images of autophagosomes and autophagolysosomes in MDA-MB-231 cells were transduced with tandem RFP-GFP-LC3B and then independently treated with aloperine (100 μM), chloroquine (25 μM), flavopereirine, or a combination of the two drugs. In the merged images, autophagosomes are presented as yellow or orange puncta (RFP-GFP-LC3B), whereas red puncta (RFP-LC3B) indicate autophagolysosomes because acidification abolishes the green fluorescence. Images were obtained using a 63× oil immersion objective.