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Representative Chromatograms used to Identify and Quantify Mitragynine. The kratom extract (100 µg/mL in methanol) was prepared and assayed as described in the methods section. (A) shows base peak chromatogram. (B) shows extracted ion chromatogram identifying chemistry with molecular formula C23H30N2O4 for mitragynine and related isomers. (C) shows extracted ion chromatogram identifying mitragynine using Targeted MS/MS transition of 399.23 to 174.09 m/z (D).
