Figure 2.

Invasiveness of GBM4 and GBM8 correlate with their pathogenicity in human patients. (a) Co-culture of GBM4 (red), GBM8 (green) and C57BL6 mouse neural progenitor cell derived spheroids (blue) in Noble agar before imaging. Scale bar 100 μm (b) Representative time-lapse images showing GBM8 preferentially invaded as single cells (white arrowheads) while GBM4 invaded in a collective manner as a group (white arrows). Images were taken 10 min apart at a 5 µm step size using confocal microscopy. Scale bar 100 μm (c) Mean velocity of GBM4 and GBM8 single cells in mouse wild-type spheroids. The data shown here are pooled from at least three experiments. Data were analyzed by Student’s t test. * P < 0.05.