Figure 3.

Immunostaining of NF-κB and quantitative evaluation analyzed by cell counting on the corneal periphery in each group. (a,b) Representative sections at 6 h after the injury are shown. As compared to the PPARβ/δ group, the vehicle group exhibited strong staining in the nucleus of inflammatory cells (see boxed area). (c,d) Representative sections at day 4 after injury are shown. Higher magnification pictures of the boxed area are also shown. NF-κB-positive inflammatory cells (black arrows) were observed in the corneal limbs. Bar, 50 μm. The number of cells stained in the nucleus was significantly lower in the PPARβ/δ group versus the vehicle group at each of the time points (e). Data are presented as mean ± SD (n = 8) ** p < 0.01.