Figure 3.

The influence of prostaglandin E₂ (PGE₂; 100, 250, 500 ng/mL) on G protein-coupled receptor 1 (GPR1) mRNA (A,C,E,G,I) and protein (B,D,F,H,J) expression in the porcine endometrium on days 10 to 11, 12 to 13, 15 to 16, and 27 to 28 of the pregnancy, and on days 10 to 11 of the estrous cycle. The gene expression was determined by quantitative real-time PCR. The protein concentration was determined by the western blotting analysis; upper panels: Representative immunoblots; lower panels: Densitometric analysis of GPR1 protein relative to actin protein. Results are reported as the means ± S.E.M. (n = 5). Bars with different superscripts differ (p < 0.05).