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. 2020 Jul 31;21(15):5502. doi: 10.3390/ijms21155502

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of coptisine on cell viability and apoptosis in Hep3B cells. Hep3B cells were treated with the indicated concentrations of coptisine for 24 h. (A) After treated with coptisine, added the a 3-(4,5-dimethylthiazol-2-yl)-2-,5-diphenyltetrazolium bromide (MTT) solution, reacted for 2 h and then detected cell viability; (B) The morphology of Hep3B cells by coptisine were observed by phase-contrast microscopy (magnification, ×50); (C) The cells were harvested and stained with annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI). After staining, cells were analyzed the apoptosis by flow cytometry. Annectin V-FITC results represent early apoptosis (lower right quadrant), and late apoptosis (upper right quadrant); (D) Statistical analysis of annexin V positive cells (** p < 0.01, and *** p < 0.001 vs. control). The results are presented as the mean ± standard deviation (SD) of three independent experiments.