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. 2020 Jul 31;21(15):5502. doi: 10.3390/ijms21155502

Figure 6.

Figure 6

Induction of apoptotic cell death via ROS production by coptisine in Hep3B cells. Cells were pretreated with or without 10 mM NAC for 1 h followed by coptisine treatment for 24 h. (A) Cell viability was measured by an MTT assay. *** p < 0.001 vs. control; (B) The apoptosis regulatory proteins were detected by Western blot analysis; (C) Comet assay was performed, and representative images were taken by a fluorescence microscope. Scale bars. 50 µm; (D) The statistical analysis of tail length. ** p < 0.01 and *** p < 0.001 vs. control; (E,F) p-γH2AX expression was assessed by Western blot analysis and fluorescence microscopy. DAPI staining was used to visualize nuclei. Scale bars, 30 µm.