Figure 1.

Genome editing in the rice OsSLR1 gene. (A) Design of single guide RNA (sgRNA) sites in the TVHYNP motif; the nuclease cleavage site is represented by the red arrow and the Protospacer Adjacent Motif (PAM) (NGG) appears in blue. DELLA protein organization representing the conserved domains. (B) Nucleotide sequence alignment by deep sequence analysis of the sgRNA target region in six mutant lines of transformed rice plants. Deletion and insertion indicated by dash and red letters, respectively. (C) Amino acid sequences of the target region in six mutant lines.