FIGURE 2.

MBTD1 is required for decidualization of immortalized human endometrial stromal cells (HESCs). (A) Levels of PRL and MBTD1 transcripts from immortalized HESCs after induction of decidualization over 6 days. (B) Representative images of transformed HESCs transfected with control or MBTD1 siRNA prior to (day 0) and after 6 days of culture in decidualization (EPC, Estrogen-MPA-cAMP) conditions. Red arrowheads indicate non-decidualized cells, and black arrowheads indicate decidualized cells. Scale bar: 200 μm. (C) Levels of MBTD1 and PRL transcripts in immortalized HESCs transfected with control or MBTD1 siRNA after 6 days of decidualization. Results are mean ± SEM from three biological replicates; **P < 0.01, ***P < 0.001, and ****P < 0.0001.