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. 2020 Aug 11;8:745. doi: 10.3389/fcell.2020.00745

FIGURE 5.

FIGURE 5

MBTD1 controls human endometrial stromal cell proliferation and cell cycle progression. (A–D) Human endometrial stromal cells (HESCs) transfected with control (A), MBTD1 (B), PGR (C), and PGR + MBTD1 siRNAs (D). After 48 h of transfection, cells were treated in decidualization media for 48 h and subjected to flow cytometry analysis. Histograms depict the percentages of cells in each phase of the cell cycle. (E) Graph depicting the distribution of cells in indicated phases of the cell cycle. (F) MTT cell proliferation assay of HESCs transfected with control siRNA or MBTD1 siRNA or PGR siRNA or MBTD1 + PGR siRNAs at indicated time points. (G) Transcript levels of MBTD1 (left panel) and PGR (right panel) from HESCs after 48 h of transfection with indicated siRNAs. Representative data from one patient sample and the experiment repeated three times with independent HES cell lines. Results represent the mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ns, non-significant.