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. 2020 Aug 11;8:745. doi: 10.3389/fcell.2020.00745

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Copyright © 2020 Chadchan, Maurya, Krekeler, Jungheim and Kommagani.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MBTD1 is not a progesterone-responsive gene in human or mouse endometrium. (A,B) Relative amounts of MBTD1 and FOXO1A mRNA (A) and MBTD1 protein with densitometric analysis (B) in human endometrial stromal cells (HESCs) treated with 1 μM MPA over 24 h. Representative data from three replicates from one patient sample are shown as mean ± SEM. GAPDH was used as a loading control. (C) The relative transcript levels of Areg (amphiregulin), Ihh (Indian hedgehog), and Il13ra2 (interleukin 13 receptor subunit alpha 2) and Mbtd1 in uteri from ovariectomized mice treated with vehicle (oil) or 1 mg progesterone (P4) for 6 h. Results represent the mean ± SEM; n = 5 mice/group. *P < 0.05, and ns, non-significant.