Figure 3.

Protective effect of glutathione (GSH) and N-acetyl-cysteine (NAC) on MCF-7 cell viability after resveratrol, curcumin and piperine treatment: Cells were treated with 50, 75, 100 and 300 µM of GSH or 0.5, 1.0, 2.0 and 5.0 mM of NAC, and 131.0 µM resveratrol (A,B), 24.5 µM curcumin (C,D) or 94.5 µM piperine (E,F) were added after 30 min,. After 24 h, cell viability was determined by the MTT assay. The results are representative of three experiments performed in triplicate ± SEM. * p < 0.05, ** p < 0.001, and *** p < 0.0001 in relation to the control.