Figure 4.

Effect of miR-96 expression on the migration and tumor sphere formation. C666-1 cells were transfected with either pre-miR-96 or anti-miR-96. (A) The migration of the transfected cells was assessed by the transwell migration assay. Upper panel: images of the migrated cells. Lower panel: quantitative analysis of the migrated cells. (B) Tumor sphere formation assay. Upper panel: images of tumor spheres in culture well of ultralow attachment plate. Lower panel: quantitative analysis of spheroid number. (C) Size distribution of tumor spheres from (B). At least three independent experiments were carried out. * p < 0.05; ** p < 0.01 compared with the corresponding control.