Figure 5.

Increased bone formation in LP zebrafish after two months of dietary treatment. Synchrotron X-ray tomographic microscopy scans reveal an increased amount of non-mineralised matrix in the vertebral body and arches of low P diet (LP) treated animals compared to controls (RP) and high P diet (HP) treated individuals. (a) Lateral view of the 10th caudal vertebral body, neural (na) and haemal (ha) arches and their spines (ns, hs, respectively). Vertebral endplates are indicated by black arrowheads. Scale bar: 100 µm. (b) Schematic representation of the measured parameters: vertebral body length, vertebral body height, length of arch plus spine. (c) Frontal view of vertebrae. Scale bar: 100 µm. (d) Virtual sagittal sections of the vertebral bodies. Scale bar: 100 µm. Non-mineralised bone: pink; mineralised bone: red.