Figure 7.

The effects of ROCK inhibitor Y27632 on cell migration and the expression of contractile marker proteins in HA-VSMCs. Cells were pretreated with Y27632 (20 μM) for 30 min and then exposed to 100 nM Ang II for 24 h. Untreated cells were used as the control. (A) Cell migration was detected by wound-healing and Transwell assays. Histograms show the quantification of the wound healing and Transwell assay results. The scar bar is 50 μm. ** p < 0.01 vs. the control group; # p < 0.05 and ## p < 0.01 vs. the Ang II-treated group (n = 3). (B) Western blot analysis of MYH11, SM22α, and smoothelin. Histograms show the ratios of MYH11 or SM22α or smoothelin to β-tubulin. * p < 0.05 vs. the control group; # p < 0.05 vs. the Ang II-treated group (n = 3).