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. 2020 Jul 29;21(15):5398. doi: 10.3390/ijms21155398

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The effects of Y27632 pretreatment on F-actin polymerization in Ang II-treated HA-VSMCs. Cells were pretreated with Y27632 (20 μM) for 30 min and then exposed to 100 nM Ang II for 24 h. Untreated cells were used as the control. (A) The ratio of F-actin to G-actin analyzed by G-actin/F-actin In Vivo Assay Kit and Western blot analysis. Histogram shows the ratio of F-actin to G-actin. * p < 0.05 vs. the control group; # p < 0.05 vs. the Ang II-treated group (n = 3). (B) Phalloidin conjugates for staining F-actin filaments. Immunofluorescence assay for F-actin (red) and nuclei stained with DAPI (blue). The scar bar is 20 μm.