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CRISPR/Cas9 mediated gene editing of AvrLm4-7 gene of Leptosphaeria maculans. (A) The mutant isolate umavr7 carries an 8 bp deletion overlapping targeted sgRNA region. (B) Alignment of deduced amino acid sequences of AvrLm4-7 with wild type isolate UMAvr7 and mutant isolate umavr7. Amino acid residues with asterisks are conserved in aligned sequences.
