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. 2020 Jul 30;21(15):5419. doi: 10.3390/ijms21155419

Figure 10.

Figure 10

Functional characterization of DoTPS10. (A–D) Subcellular localization of DoTPS10 fused with yellow fluorescent protein (YFP) and transiently expressed in A. thaliana protoplasts. Bars = 5 μm. (E) SDS-PAGE analysis of DoTPS10 recombinant protein expressed in Escherichia coli BL21. Lanes M, 1, 2, and 3 indicate marker, pET32a, crude DoTPS10 protein, and purified DoTPS10 protein, respectively. Red arrow indicates target protein. (F–G) Gas chromatograms of products yielded by DoTPS10 using GPP as a substrate. (H) Mass spectrum of linalool was identical to the mass spectrum of the linalool standard.