Figure 5.
Effect of tomatine on melanoma-dependent vascular disruption of N-MVECs (microvascular endothelial cells, human dermal microvascular endothelial cells). Cells (12 × 103) were plated on 10–12 mg/mL matrigel-coated tissue culture wells, treated immediately with melanoma cells CM, and after 3 h with tomatine (0.5 and 1 μM in terms of α-tomatine). The cells were photographed 9 h after plating. Six to nine photographic fields from three plates were scanned for each point, considering as 100% the area occupied by untreated N-MVECs. The results shown are representative of three different experiments.