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Schematic diagram of the study design (a) and detailed methods (b) to isolate salivary small extracellular vesicles (sEV) by ultracentrifuge (UC) and size exclusion chromatography (SEC). DiO: 3,3′-Dioctadecyloxacarbocyanine perchlorate; qMSP: quantitative methylation-specific real-time PCR; SN: supernatant.
