Figure 2.

Identification of the injection point via MR image and histological stains. (A) Representative T2 weighted MR images acquired at post 3 weeks from C57VCI mice from the MEM and MSC groups. L indicates left and R indicates the right hemisphere of the mouse brain. The yellow solid arrow indicates hyperintense signals that appear as a vertical streak from the cortex to the hippocampus. The green solid arrow indicates minor damage to the cortex due to the penetration of the mouse parenchyma by the Hamilton syringe. The location of each of the coronal MR sections is illustrated by the sagittal section of the mouse brain (right): (1) forceps minor of corpus callosum (+1.70 mm anterior to bregma), (2) external capsule of the corpus callosum (+0.62 mm anterior to bregma), (3) the injection point (lateral ventricles) and the caudate putamen can be visualized (−0.22 mm posterior to bregma), and (4) the hippocampal fimbria and hippocampus can be detected (−1.82 mm posterior to the bregma). An infarct is detected in the left caudate putamen (section #3) of the representative C57VCI mouse from the MEM group and infarcts in the right anterior commissure (section #1) and corpus callosum (sections #2 and 3) are detected from the representative C57VCI mouse from the MSC group. (B) Similar histological sections that matched the MRI slices where hypointense or hyperintense signals were identified were stained with hematoxylin and eosin (H&E). Puncture of the cortex (solid black arrowheads) via the insertion of the Hamilton syringe was exhibited from the H&E stains. Scale bar = 2 mm.