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. 2020 Jul 27;21(15):5332. doi: 10.3390/ijms21155332

Figure 7.

Figure 7

Inhibition of BMP2 and β-catenin signaling attenuates osteoblast differentiation using TMARg treatment. (A) The cells were seeded onto 48-well plates and were cultured in OS with TMARg (30 µM) in the absence or presence of Dkk-1 (0.5 µg/mL) and noggin (10 µg/mL) for 5 days. ALP activity was measured by using ALP activity assays. (B) The cells were seeded onto 24-well plates and differentiated for 14 days. Mineralized nodule formation was assessed using ARS staining. (C) BMP2 was immunostained with rabbit anti-BMP2 antibody, followed by Alexa-Fluor 488-conjugated secondary antibody (green). Then, the cells were counterstained with DAPI (blue). The bottom panels show the magnification of the merged images. The arrow indicates the magnified region. Scale bar: 50 µm. The data are representative of the results of three independent experiments. Data represent the means ± SEMs of experiments. *, p < 0.05 was considered significantly different, compared to the control. #, p < 0.05 was considered significantly different, compared to the OS. $, p < 0.05 was considered significantly different, compared to the OS + TMARg.