Figure 3.

Effect of CAC secretome on endothelial colony-forming cells (ECFC) angiogenic potential. ECFC were treated (n:3 per condition) with either serum free-EBM-2 medium, basal condition (B50, B100); CAC control secretome (C50, C100) or the secretome of CAC pre-incubated with AP factors (AP50, AP100), at different concentrations (50 and 100 ng/μL), for 24 and 48 h. In addition, ECFC were incubated with FGF (angiogenesis activator) and SR (inhibitor) as positive and negative angiogenic controls. Graphical results indicate (A) number of meshes, (B) total meshes area (pixels²), (C) number of segments, and (D) total segments length (pixels). (E) Representative image of all conditions tested at 24 and 48 h are shown. Images were taken with an inverted phase-contrast microscope, under 40× magnification. Data were presented as mean ±SE. The most relevant significant changes are shown (* p < 0.05; ** p < 0.01; *** p < 0.001), while the entire set of p-values < 0.05 has been included in Supplementary Table S3. Significant differences between conditions (—); Significant differences vs. the activator (FGF) (----).